The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
The Drosophila big brain (bib) protein functions critically in the determination of neuroblasts in the embryonic ectoderm and many other cell types. bib is a member of the MIP family of transmembrane channel proteins. The conserved channel domain of bib is flanked by amino- and carboxy-terminal cytoplasmic domains of unique sequence, which comprise over two-thirds of the protein. To determine whether...
Mouse ES cells with a null mutation of the known DNA methyltransferase retain some residual DNA methylation and can methylate foreign sequences de novo. We have used bisulfite genomic sequencing to examine the sequence specificity and distributions of methylation of a hypermethylated CG island sequence, mouse A-repeats. There were 13 CG dinucleotides in the region examined, 12 of which were methylated...
Streptococcus mutans, along with many other Gram-positive bacteria produce small antibacterial peptides called bacteriocins. Bacteriocins elaborated by S. mutans, termed mutacins, may provide a selective force necessary for initial or sustained colonization in dental plaque by this major dental pathogen. Previously, we purified and characterized mutacin II, the first lantibiotic found in S. mutans...
The I-PpoI endonuclease is encoded by a group I intron found in the slime mold Physarum polycephalum. To initiate homing of its encoding intron, I-PpoI catalyzes a specific double-stranded break within a 15-bp recognition site. The high substrate specificities of I-PpoI and other homing endonucleases make these enzymes valuable tools for genomic mapping and sequencing. Here, we report on the ability...
We have isolated the gene encoding the beta subunit of mitochondrial processing peptidase (β-MPP) from the shiitake mushroom Lentinula edodes. It is a nuclear gene with two small introns. Comparison with known β-MPP genes revealed that the L. edodes gene is most closely related with that from Neurospora crassa, with 60.8% identities and 87% similarity in the amino-acid sequences. The deduced L. edodes...
The human Y-box protein (YB-1) is a member of a family of DNA-binding proteins contain a highly conserved cold shock domain. The genomic organization of the human YB-1 gene was determined from five overlapping genomic clones that encompassed all exons of the gene. Sequence analysis of these clones revealed that human YB-1 spans approximately 19kb of genomic DNA and contains eight exons. The cold shock...
Antimicrobial peptides are an abundant and diverse component of animal innate immunity. Within mammalian species, defensins and cathelicidins are the two principal antimicrobial peptide families. We identified and sequenced ten new sheep genes which encode potential antimicrobial peptides including two β-defensins and eight cathelicidins. We mapped the two-exon β-defensin genes to sheep chromosome...
A 2,585bp chromosomal DNA segment of Ralstonia eutropha JMP134 (formerly: Alcaligenes eutrophus JMP134) which contains a gene cluster encoding part of the modified ortho-cleavage pathway encodes a putative transport protein for 4-methylmuconolactone, a novel 4-methylmuconolactone methylisomerase and methylmuconolactone isomerase. The putative 4-methylmuconolactone transporter, a protein with a calculated...
A cDNA library was constructed from mRNA of the rhesus monkey kidney cell line, FRHK, and the cDNA sequence for an FRHK glutathione S-transferase (GST) Pi was determined using a RACE method. This represents the first full-length monkey GST Pi sequence to be cloned and determined. The similarity to the human GST Pi was found to be extensive (more than 97%), the deduced protein differing only in six...
We identified a cDNA encoding a metallothionein (MT)-like protein from a cDNA library of rice endosperm. This cDNA (ricMT) encoded an open reading frame of 80 amino acids, with two cysteine-rich domains at the amino-terminus and carboxy-terminus, respectively. The deduced amino acid sequence was homologous to those of class I MT-like proteins. Southern blot analysis revealed that the gene exists at...
A novel P-type ATPase gene, Saccharomyces cerevisiae PMR1 homologue (YlPMR1), has been cloned and sequenced in the yeast, Yarrowia lipolytica. The putative gene product has 928 amino acids with a calculated molecular mass of 100 050Da and a pI of 5.15. The deduced amino-acid sequence analysis demonstrated that the cloned gene product contains all 10 of the conserved regions in P-type ATPases and...
We have molecularly cloned a cDNA encoding a new Rel-related protein in Xenopus laevis. The product is most homologous to mammalian p100-NFκB2. Furthermore, the putative protein kinase A-phosphorylation site (RRPS), which is found in most of the Rel family proteins and is replaced by KRKR in mammalian p100, is also replaced by KRKK in our clone, indicating that our cDNA most likely encodes the Xenopus...
Two novel cellular disintegrin genes (termed ADAM 6d and ADAM 6e: Gen Bank accession numbers U82750 and U82751) were isolated and characterised from a rabbit testicular cDNA library. The cDNAs have open reading frames encoding for proteins of 731 and 730 amino acids, respectively. They share an amino-acid homology of greater than 89% and a nucleotide base matching of 94% in both the coding and non-coding...
The gene encoding a 31-kDa major protein (Pap31) associated with the bacteriophage harbored in Bartonella henselae was cloned and sequenced. Analysis of the resulting sequence revealed an open reading frame of 837 nucleotides coding for a protein of 279 amino acids. pap31 was then subcloned downstream of the lacZ promoter in pUC19. pap31 was amplified by polymerase chain reaction, and the linear amplicon...
A highly conserved laminin receptor processed pseudogene (LAMRL5) that has been isolated from a fetal brain cDNA library is described. The pseudogene is a complete copy (97.9% identical) of the transcribed laminin receptor (LAMR1) with all the introns precisely removed. The sequence has direct repeats of 18bp at either end. It has an 885 nucleotide open reading frame from the start methionine codon...
The GTPase cycle of Rab/Ypt proteins is strictly controlled by several classes of regulators to ensure their proper roles in membrane traffic. GDP dissociation inhibitor (GDI) is known to play essential roles in regulating nucleotide states and subcellular localizations of Rab/Ypt proteins. To obtain further knowledge on this regulator molecule in plants, we isolated and characterized two genes of...
We report the utility of random amplified polymorphic DNA (RAPD) analysis for identifying subtle genomic alterations in meningiomas and gliomas by comparing the DNA band profile of tumor vis-a-vis its constitutional counterpart. Twenty out of the 29 decanucleotide GC-rich random primers utilized for the RAPD analysis of meningiomas revealed alteration(s) in the tumor genome. In gliomas, changes were...
ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We performed Northern blot experiments using sequences from...
A DNA fragment from Pseudomonas fluorescens DSM50106 containing the genes for the uptake and utilization of mannitol, arabitol and glucitol was cloned in Escherichia coli and sequenced. Seven open reading frames (mtlEFGKDYZ) were identified on the 10031bp fragment. The deduced amino acid sequences of the first four open reading frames (mtlEFGK) revealed significant similarity to the components of...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.